Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Binding of dgo to GST-dsh is inhibited by addition of His-tagged pk, suggesting competitive binding to the dsh bPDZ domain.

Binding of radiolabeled, in vitro translated dgo to GST-dsh was tested in the presence of increasing amounts of recombinant His-tagged pk.