FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mir-14, EcR
FlyBase ID
FBig0000104741
Interaction Network
Interactions Browser links
mir-14 network
EcR network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0201357-1.miR-mRNA.LUC
Reference
Varghese and Cohen, 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
mir-14 

mir-14

bait
mir-14 
EcR 

EcR

prey
EcR 
EcR 
EcR 
EcR 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
EcR 
3-prime UTR
sufficient binding region
EcR 
mir-14 target site 488
necessary binding region
GGAAGAGAGAAGAGAAUAAAGAUUGU
EcR 
mir-14 target site 729
necessary binding region
UGGACUGAU
EcR 
mir-14 target site 962
necessary binding region
UGAAAUGAAAGUGAUUGGA
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

(Varghese and Cohen, 2007)

luciferase reporter assay

(Varghese and Cohen, 2007)
FBrf0201357-2.miR-mRNA.GFP
Reference
Varghese and Cohen, 2007
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-14 

mir-14

bait
EcR 

EcR

prey
EcR 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
EcR 
3-prime UTR
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs; bait produced from transgenic construct; prey produced from transgenic construct.

(Varghese and Cohen, 2007)

GFP reporter assay

(Varghese and Cohen, 2007)
FBrf0201357-3.miR-mRNA.IHC
Reference
Varghese and Cohen, 2007
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-14 

mir-14

bait
EcR 

EcR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs; bait produced from transgenic construct; prey produced from endogenous gene.

(Varghese and Cohen, 2007)

Overexpression of mir-14 reduces EcR protein levels.

(Varghese and Cohen, 2007)
FBrf0201357-4.miR-mRNA.WB
Reference
Varghese and Cohen, 2007
Description
physical association
Assay
Collection
Source/Stage
organism / pupal stage P6
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-14 

mir-14

bait
EcR 

EcR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was pupae from mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Varghese and Cohen, 2007)

Loss of mir-14 leads to upregulation EcR protein levels.

(Varghese and Cohen, 2007)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)