The PLA signal develops only if primary antibodies to the two proteins are in close proximity to each other (within tens of nanometers).

Source was fixed larval body wall of wild-type fly line; proteins produced from endogenous genes.

Source was adult heads of wild-type or mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

The futsch-Tub interaction was curated to the three Dmel alpha-tubulins with highest conservation to the epitope described in pmid:2441392.