Top2 was purified from S2 cells in the presence of phosphatase inhibitors to preserve post-translational modifications, then used for in vitro binding assay.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey purified from S2 nuclear extract.

Top2 was purified from S2 cells in the presence of phosphatase inhibitors to preserve post-translational modifications, then used for in vitro binding assay.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey purified from S2 nuclear extract.

Interaction in vitro; bait chemically synthesized and labeled in vitro; prey produced as a recombinant fusion protein in bacterial system.

The interaction of mus101 with Top2 peptide requires phosphorylation of Top2 residues S1428 and S1443.

Interaction in vitro; bait chemically synthesized and labeled in vitro; prey produced as a recombinant fusion protein in bacterial system.

mus101 binds Top2 with Kd = 0.57 uM. This interaction requires phosphorylation of Top2 residues S1428 and S1443.