FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
MAPk-Ak2, IscU
FlyBase ID
FBig0000104841
Interaction Network
Interactions Browser links
MAPk-Ak2 network
IscU network
Reported Interactions
FBrf0226741-1.coIP.WB
Reference
Tian et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
IscU 

dIscU

bait

FLAG tag

MAPk-Ak2 

dMK2

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tian et al., 2014)
FBrf0226741-2.coIP.WB
Reference
Tian et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
MAPk-Ak2 

dMK2

bait

FLAG tag

IscU 

dIscU

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tian et al., 2014)
FBrf0226741-3.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
MAPk-Ak2 

dMK2

enzyme

GST tag

MAPk-Ak2 

dMK2

enzyme

GST tag

IscU 

dIscU

enzyme target

GST tag, P[32] label

IscU 

dIscU

enzyme target

GST tag, P[32] label

IscU 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)