FB2025_04 , released October 2, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mir-2b-2, grim
FlyBase ID
FBig0000104882
Interaction Network
Interactions Browser links
mir-2b-2 network
grim network
Reported Interactions
FBrf0167987-4.miR-mRNA.GFP
Reference
Stark et al., 2003
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-2b-2 

miR-2b

bait
grim 

grim

prey
grim 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
grim 
3-prime UTR
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs of transgenic fly line; bait produced from transgenic construct; prey produced from transgenic reporter construct.

(Stark et al., 2003)

GFP reporter assay.

(Stark et al., 2003)
FBrf0187230-26.miR-mRNA.GFP
Reference
Leaman et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 13
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-2b-2 

miR-2/13

bait
grim 

grim

prey
grim 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
grim 
3-prime UTR
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

In vitro synthesized miRNAs were injected into embryos before cellularization, and effects on the reporter were assessed at 10 hr AEL.

(Leaman et al., 2005)

GFP reporter assay.

(Leaman et al., 2005)

2\'O-methyl oligoribonucleotides antisense to mir-2b-2 leads to derepression of the grim GFP reporter.

(Leaman et al., 2005)

Source was embryos of transgenic fly line; bait synthesized in vitro; prey produced from transgenic reporter construct.

(Leaman et al., 2005)
FBrf0188411-1.miR-mRNA.GFP
Reference
Brennecke et al., 2005
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-2b-2 

mir-2b

bait
grim 

grim

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs of transgenic fly line; bait produced from transgenic construct; prey produced from transgenic reporter construct.

(Brennecke et al., 2005)

GFP reporter assay.

(Brennecke et al., 2005)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)