FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
e(y)2, Orc5
FlyBase ID
FBig0000104944
Interaction Network
Interactions Browser links
e(y)2 network
Orc5 network
Reported Interactions
FBrf0232523-18.coIP.WB
Reference
Kopytova et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
e(y)2 

ENY2

bait
Orc5 

Orc5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Embryonic nuclear extract was treated with DNase and RNase.

(Kopytova et al., 2016)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kopytova et al., 2016)
FBrf0232523-38.coIP.WB
Reference
Kopytova et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Orc5 

Orc5

bait
e(y)2 

ENY2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Embryonic nuclear extract was treated with DNase and RNase.

(Kopytova et al., 2016)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kopytova et al., 2016)
FBrf0232523-6.coIP.MS
Reference
Kopytova et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
e(y)2 

ENY2

bait
Orc5 

Orc5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Crude nuclear extract was subjected to several conventional chromatographic steps and gel filtration to purify the 700 kDa TREX-2 complex. This purified fraction was then used for immunoprecipitation.

(Kopytova et al., 2016)

The interaction was not affected by treatment with DNase or RNase.

(Kopytova et al., 2016)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kopytova et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)