Deletion of mir-310, mir-311, mir-312 and mir-313 (using Df(2R)KT40) results in increased levels of Dg mRNA, particularly the long 3\'UTR isoforms.

Source was adult heads of mutant fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Overexpression of mir-310, mir-311, mir-312 and mir-313 in ovarian follicle cell clones reduces Dg protein levels.

Source was adult female follicle cells of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

Luciferase reporter assay.

Source was S2R+ cell line; bait produced from transfected construct; prey produced from transfected reporter construct.