In vitro synthesized miRNAs were injected into embryos before cellularization, and effects on the reporter were assessed at 10 hr AEL.

GFP reporter assay.

2\'O-methyl oligoribonucleotides antisense to mir-6-1 leads to derepression of the skl GFP reporter.

Source was embryos of transgenic fly line; bait synthesized in vitro; prey produced from transgenic reporter construct.

Source was larval wing discs of transgenic fly line; bait produced from transgenic construct; prey produced from transgenic reporter construct.

GFP reporter assay.