FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
thoc5, e(y)2
FlyBase ID
FBig0000104972
Interaction Network
Interactions Browser links
thoc5 network
e(y)2 network
Reported Interactions
FBrf0209575-15.coIP.WB
Reference
Kopytova et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
thoc5 

THOC5

bait
e(y)2 

ENY2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Extract was treated with DNase and RNase.

(Kopytova et al., 2010)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kopytova et al., 2010)
FBrf0209575-4.coIP.MS
Reference
Kopytova et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
e(y)2 

ENY2

bait
thoc5 

THOC5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Crude nuclear extract was subjected to several conventional chromatographic steps and gel filtration to purify the 2.0 MDa e(y)2-containing THO complex. This purified fraction was then used for e(y)2 immunoprecipitation.

(Kopytova et al., 2010)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kopytova et al., 2010)
FBrf0209575-8.coIP.WB
Reference
Kopytova et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
e(y)2 

ENY2

bait
thoc5 

THOC5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Extract was treated with DNase and RNase.

(Kopytova et al., 2010)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kopytova et al., 2010)
FBrf0211509-1.CH.WB
Reference
Gurskiy et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
e(y)2 

ENY2

neutral component
e(y)2 

ENY2

neutral component
e(y)2 

ENY2

neutral component
thoc5 

THOC5

neutral component
thoc5 

THOC5

neutral component
thoc5 

THOC5

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; proteins derived from endogenous genes.

(Gurskiy et al., 2010)
FBrf0211509-13.coIP.WB
Reference
Gurskiy et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
thoc5 

THOC5

bait
e(y)2 

ENY2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gurskiy et al., 2010)
FBrf0211509-5.coIP.MS
Reference
Gurskiy et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
e(y)2 

ENY2

bait
thoc5 

THOC5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was fraction of wild-type embryonic nuclear extract; bait produced from endogenous gene; prey produced from endogenous gene.

(Gurskiy et al., 2010)
FBrf0211509-6.coIP.WB
Reference
Gurskiy et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
e(y)2 

ENY2

bait
thoc5 

THOC5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gurskiy et al., 2010)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)