FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Orc2, Rbf
FlyBase ID
FBig0000104982
Interaction Network
Interactions Browser links
Orc2 network
Rbf network
Reported Interactions
FBrf0134797-4.PD.WB
Reference
Bosco et al., 2001
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Orc2 

ORC2

bait
Rbf 

RBF

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Bosco et al., 2001)
FBrf0205842-1.coIP.WB
Reference
Ahlander et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Orc2 

Orc2

bait
Rbf 

Rbf1

prey

V5 tag

Rbf 
Rbf 
Rbf 
Rbf 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rbf 
Rbf1 N-terminal fragment
sufficient binding region
aa 1-330
Rbf 
Rbf1 C-terminal fragment
sufficient binding region
aa 345-845
Rbf 
Rbf1 C-terminal fragment
necessary binding region
aa 797-845
Rbf 
Rbf1 N-terminal fragment
necessary binding region
aa 150-330
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ahlander et al., 2008)

Orc2 did not interact with Rbf2 in similar experiments.

(Ahlander et al., 2008)
FBrf0205842-3.FRET.FM
Reference
Ahlander et al., 2008
Description
physical association
Assay
Collection
Source/Stage
salivary gland / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rbf 

Rbf1

fluorescence donor

RFP tag

Rbf 

Rbf1

fluorescence donor

RFP tag

Rbf 
Rbf 

Rbf1

fluorescence donor

RFP tag

Rbf 
Orc2 

Orc2

fluorescence acceptor

GFP tag

Orc2 

Orc2

fluorescence acceptor

GFP tag

Orc2 

Orc2

fluorescence acceptor

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rbf 
Rbf1 N-terminal fragment
sufficient binding region
aa 1-345
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval salivary gland of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Ahlander et al., 2008)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)