Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).
Two-hybrid system: yeast LexA-BD/B42-AD
Source was larval body wall muscle of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
multiple alanine substitutions, coordinates relative to gukh-PA
multiple alanine substitutions, coordinates relative to gukh-PA
multiple alanine substitutions, coordinates relative to gukh-PA
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
R822A, coordinates relative to dlg1-PG
D830G, coordinates relative to dlg1-PG
Y831A, coordinates relative to dlg1-PG
Y855A, coordinates relative to dlg1-PG
Two-hybrid system: yeast GAL4-BD/GAL4-AD.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Frameshift mutation that deletes the F domain, coordinates relative to dlg1-PG
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
