FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Cad96Ca, Src64B
FlyBase ID
FBig0000105080
Interaction Network
Interactions Browser links
Cad96Ca network
Src64B network
Reported Interactions
FBrf0224726-3.Y2H
Reference
Tsarouhas et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cad96Ca 

Stit

bait

fused to LexA DNA-binding domain

Cad96Ca 
Src64B 

Src64B

prey

fused to activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cad96Ca 
intracellular domain
sufficient binding region
aa 337-773
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/AD

(Tsarouhas et al., 2014)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Tsarouhas et al., 2014)

16 of 185 Cad96Ca-interacting clones represented Src64B.

(Tsarouhas et al., 2014)
FBrf0224726-7.PD.WB
Reference
Tsarouhas et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Src64B 

Src64B

bait

GST tag

Src64B 
Cad96Ca 

Stit

prey

V5 tag

Cad96Ca 
Cad96Ca 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Src64B 
SH2 domain
sufficient binding region
Cad96Ca 
kinase domain
mutation disrupting interaction
aa 504

K504A

Cad96Ca 
phosphorylation site Y762
mutation disrupting interaction
aa 762

Y762F

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Tsarouhas et al., 2014)

Src64B does not interact with kinase-dead Cad96Ca.

(Tsarouhas et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)