Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
"5D" construct, S348D, S358D, T379D, S387D and S392D, coordinates relative to yrt-PA
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.
Phosphorylation of the yrt FA domain inhibits binding by aPKC, since the phosphomimetic "5D" mutation disrupts binding.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
