Luciferase reporter assay.

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

Mutation of mir-279 increases nuclear Stat92E protein levels in border cells. Expression of a mir-279 sponge has a similar effect on Stat92E reporter expression.

Source was adult female stage10 egg chambers of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.