FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mir-960, smo
FlyBase ID
FBig0000105290
Interaction Network
Interactions Browser links
mir-960 network
smo network
Reported Interactions
FBrf0221156-1.miR-mRNA.PCR
Reference
Gao et al., 2013
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-960 

miR-960

bait
smo 

Smo

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

(Gao et al., 2013)

Overexpression of mir-960 reduces smo protein levels.

(Gao et al., 2013)
FBrf0221156-4.miR-mRNA.GFP
Reference
Gao et al., 2013
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mir-960 

miR-960

bait
smo 

smo

prey
smo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
3-prime UTR
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs of transgenic fly line; bait produced from transgenic construct; prey produced from transgenic reporter construct.

(Gao et al., 2013)

GFP reporter assay.

(Gao et al., 2013)
FBrf0221156-7.miR-mRNA.LUC
Reference
Gao et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
mir-960 

miR-960

bait
smo 

smo

prey
smo 
smo 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
smo 
3-prime UTR
sufficient binding region
smo 
mir-960 target site
necessary binding region
GAAUACUC
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

(Gao et al., 2013)

Luciferase reporter assay.

(Gao et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)