FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Plp
FlyBase ID
FBig0000105295
Interaction Network
Interactions Browser links
Plp network
Reported Interactions
FBrf0229262-6.Y2H
Reference
Richens et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Plp 

D-Plp

bait

fused to GAL4 DNA-binding domain

Plp 

D-Plp

prey

fused to GAL4 activation domain

Plp 
Plp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Plp 
middle region M1
sufficient binding region
aa 904-1202

construct "M1", coordinates relative to Plp-PF

Plp 
middle region M4-C1
sufficient binding region
aa 1872-2471

construct "M4-C1", coordinates relative to Plp-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Richens et al., 2015)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Richens et al., 2015)

Various regions of Plp homodimerize in this assay.

(Richens et al., 2015)
FBrf0233282-121.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Plp 

PLP

bait

fused to GAL4 DNA-binding domain

Plp 

PLP

prey

fused to GAL4 activation domain

Plp 
Plp 
Plp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Plp 
region F3
sufficient binding region
aa 1377-1811

coordinates relative to Plp-PF

Plp 
region F2
sufficient binding region
aa 584-1376

coordinates relative to Plp-PF

Plp 
C-terminal region
sufficient binding region
aa 2539-2895

coordinates relative to Plp-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)

Weak interaction. Region F3 weakly homodimerizes. Weak interaction of region F2 with the Plp C-terminal region.

(Galletta et al., 2016)
External Crossreferences and Linkouts ( 0 )
References (2)