FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
arm, NitFhit
FlyBase ID
FBig0000105378
Interaction Network
Interactions Browser links
arm network
NitFhit network
Reported Interactions
FBrf0232945-1.coIP.WB
Reference
Mittag et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
arm 

Arm

bait

FLAG tag

NitFhit 

dNitFhit

prey

Myc tag

NitFhit 
NitFhit 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
NitFhit 
Nit domain
sufficient binding region
NitFhit 
Fhit domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK-293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Mittag et al., 2016)
FBrf0232945-2.PD.MW
Reference
Mittag et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
arm 

Arm

bait

GST tag

NitFhit 

dNitFhit

prey

MBP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Mittag et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)