Source was larval wing discs of transgenic fly line; bait produced from transgenic construct; prey produced from transgenic reporter construct.
Overexpression of mir-305 suppresses p53 reporter construct expression, while p53 reporter expression in increased in mir-305 mutant clones. These effects are dependent on the prediced mir-305 target site.
GFP reporter assay.
Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.
Luciferase reporter assay.