FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
spd-2
FlyBase ID
FBig0000105417
Interaction Network
Interactions Browser links
spd-2 network
Reported Interactions
FBrf0233282-132.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
spd-2 

Spd2

bait

fused to GAL4 DNA-binding domain

spd-2 

Spd2

prey

fused to GAL4 activation domain

spd-2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
spd-2 
C-terminal region
sufficient binding region
aa 664-1146

coordinates relative to spd-2-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)
FBrf0242776-2.coIP.WB
Reference
Gambarotto et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
spd-2 

Spd-2

bait

GFP tag

spd-2 

Spd-2

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gambarotto et al., 2019)
External Crossreferences and Linkouts ( 0 )
References (2)