FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mbf1, Jra
FlyBase ID
FBig0000105500
Interaction Network
Interactions Browser links
mbf1 network
Jra network
Reported Interactions
FBrf0179772-1.PD.WB
Reference
Jindra et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mbf1 

MBF1

bait

GST tag

Jra 

D-Jun

prey

His tag

Jra 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Jra 
bZIP domain
sufficient binding region
aa 207-279

coordinates relative to Jra-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Jindra et al., 2004)

mbf1 binds to the Jra bZIP domain, but not the kay bZIP domain.

(Jindra et al., 2004)
FBrf0179772-2.coIP.WB
Reference
Jindra et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Jra 

D-Jun

bait

His tag

mbf1 

MBF1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jindra et al., 2004)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)