Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast LexA-BD/B42-AD
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic adult extract.
Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from tagged transfected construct in HEK293T cell lysate.
Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.
"mutA" construct, RKRYQ to AAAYA, coordinates relative to Khc-PA
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.
