Two-hybrid system: yeast LexA-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Point mutations in the first two copies of the CAAGR repeats of the intronic splicing silencer of the tra2 pre-mRNA reduce tra2 binding only partially, but mutations in all five copies of repeats eliminated tra2 cross-linking.

Source was nuclear extract of S2 cell line; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in baculovirus and insect cell system.