FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Ptp61F, Hem
FlyBase ID
FBig0000105990
Interaction Network
Interactions Browser links
Ptp61F network
Hem network
Reported Interactions
FBrf0215460-3.coIP.MS
Reference
Ku et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Ptp61F 

dPTP61F

bait

HA tag

Ptp61F 
Ptp61F 
Hem 

Kette

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ptp61F 
N-terminal region
sufficient binding region
aa 1-339

coordinates relative to Ptp61F-PA

Ptp61F 
catalytic domain residue D203
mutation increasing interaction
aa 203

D203, coordinates relative to Ptp61F-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

An enzymatically inactive version of the Ptp61F was used to trap substrates.

(Ku et al., 2009)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Ku et al., 2009)
FBrf0215460-4.coIP.WB
Reference
Ku et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Ptp61F 

PTP61F

bait

HA tag

Ptp61F 
Hem 

Kette

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ptp61F 
catalytic domain residue D203
mutation increasing interaction
aa 203

D203, coordinates relative to Ptp61F-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ku et al., 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)