Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast LexA-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from ovary cDNA expression library).
D83K; coordinates relative to cuff-PA
R762E; coordinates relative to del-PA
D83K; coordinates relative to del-PA
Source was live S2R+ cells; proteins produced from transfected constructs.
Bait was fused to a pleckstrin domain with a tag. It removed nuclear proteins to the cytoplasm. Interaction was detected as movement from nuclear proteins to the cytoplasm.
Source was live S2R+ cells; proteins produced from transfected constructs.
Bait was fused to a mitochondial anchoring tag. Interaction was determined by colocalization.
