FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
eIF4EHP, bcd
FlyBase ID
FBig0000106260
Interaction Network
Interactions Browser links
eIF4EHP network
bcd network
Reported Interactions
FBrf0187220-1.coIP.WB
Reference
Cho et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4EHP 

d4EHP

bait
bcd 

Bcd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Cho et al., 2005)
FBrf0187220-2.coIP.WB
Reference
Cho et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
bcd 

Bcd

bait

FLAG tag

bcd 
bcd 
eIF4EHP 

d4EHP

prey
eIF4EHP 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eIF4EHP 
conserved tryptophan residue
mutation disrupting interaction
aa 85

W85F, coordinates relative to eIF4EHP-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Cho et al., 2005)

A mutation in the mRNA cap binding region of eIF4EHP did not affect interaction with bcd.

(Cho et al., 2005)
FBrf0187220-3.coIP.WB
Reference
Cho et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eIF4EHP 

d4EHP

bait
bcd 

Bcd

prey
bcd 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bcd 
eIF4E binding site
mutation disrupting interaction
aa 68aa 73

Y68A, aa coordinates relative to the PG isoform of Bcd.

L73R, aa coordinates relative to the PG isoform of Bcd.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Cho et al., 2005)

Two other point mutations of bcd, Y66A and Y72A, were tested that did not affect bcd interaction with eIF4EHP.

(Cho et al., 2005)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)