Two-hybrid system: yeast GAL4-BD/GAL4-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Trimeric complex containing Mon1, Ccz1 and CG8270 captured as a series of three binary interactions. Mon1 and Ccz1 also form a dimer.

Interaction in vitro; proteins produced as recombinant fusion proteins in a baculovirus and Sf21 cell system.

Mon1 and Ccz1 can form a dimer in solution or a trimer with Bulli.

Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus and Sf21 cell system.

Mon1-Ccz1-Bulli trimer captured as three pairwise interactions.

Interaction in vitro; proteins produced as recombinant fusion proteins in baculovirus and insect cell system.