eIF4B does not bind to pAbp in this experiment.

Embryonic extract was treated with RNase to demonstrate that the interaction is RNA independant.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

Estimated molecular mass consistent with a complex between Unr and pAbp.

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system; RNA chemically synthesized.

Unr ncCSD8 domain interacts with pAbp RRM3 domain; Unr CSD12 domain interacts with pAbp RRM2 and linker PABP domains. NMR spectra show competitive binding between an A8-mer RNA and Unr CSD789 to pAbp RRM3.