FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
spn-F, Dhc64C
FlyBase ID
FBig0000106559
Interaction Network
Interactions Browser links
spn-F network
Dhc64C network
Reported Interactions
FBrf0228877-5.coIP.WB
Reference
Otani et al., 2015
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
spn-F 

Spn-F

bait
Dhc64C 

DHC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Otani et al., 2015)
FBrf0228877-6.PD.WB
Reference
Otani et al., 2015
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
spn-F 

Spn-F

bait

GST tag

spn-F 
Dhc64C 

DHC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
spn-F 
Dynein-interacting region
sufficient binding region
aa 274-364

"C2" construct, coordinates relative to spn-F-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type ovarian extract.

(Otani et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)