FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
glo, nanos
FlyBase ID
FBig0000106581
Interaction Network
Interactions Browser links
glo network
nanos network
Reported Interactions
FBrf0213495-1.coIP.PCR
Reference
Andrews et al., 2011
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
glo 

Glo

bait

EGFP tag

nanos 

nos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult ovary extract; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Andrews et al., 2011)
FBrf0235193-1.EMSA.A
Reference
Tamayo et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
glo 

glo

bait

His tag

glo 
glo 
nanos 

nos

prey

[32]P label

nanos 
nanos 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
glo 
RRM1 and RRM2 domains
sufficient binding region
aa 1-224

coordinates relative to glo-PA

glo 
RRM1 and RRM2 domains
mutation decreasing interaction
aa 58,155aa 52,149

W58A,Y155A coordinates relative to glo-PA

R52A,K149A coordinates relative to glo-PA

nanos 
glo-binding site
mutation decreasing interaction
AGCUGGGUU

AGCUGGGUU to AGCUGAG

nanos 
TCEI
sufficient binding region
GAGAUCCAGCUCUGGAAUAUUCCGAUCAAAGCUGGGUU
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

(Tamayo et al., 2017)

Study shows two glo binding domains in nos RNA, a G-tract in TCEI and the UA-rich TCEIII stem loop.

(Tamayo et al., 2017)
FBrf0257509-1.coIP.PCR
Reference
Warden et al., 2023
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
glo 

Glo

bait

GFP tag

glo 
glo 
glo 
nanos 

nos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
glo 
RRM1 domain
mutation disrupting interaction
aa 47-138
glo 
RRM2 domain
mutation disrupting interaction
aa 144-233
glo 
RRM3 domain
mutation disrupting interaction
aa 477-558
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Warden et al., 2023)

nanos-glo interaction requires any two out of the three RRM domains to be intact.

(Warden et al., 2023)
FBrf0257509-2.NMR
Reference
Warden et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
glo 

Glo

neutral component
glo 

Glo

neutral component
glo 
glo 

Glo

neutral component
glo 
nanos 

nos

neutral component
nanos 

nos

neutral component
nanos 

nos

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
glo 
RRM1 and 2 domains
sufficient binding region
aa 1-233
 
TCEI and III domains
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system; RNA chemically synthesized.

(Warden et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)