FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
unc-13, Rbp
FlyBase ID
FBig0000106590
Interaction Network
Interactions Browser links
unc-13 network
Rbp network
Reported Interactions
FBrf0233590-1.Y2H
Reference
Böhme et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rbp 

RBP

bait

fused to LexA DNA-binding domain

Rbp 
Rbp 
unc-13 

Unc13A

prey

fused to GAL4 activation domain

unc-13 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
unc-13 
Unc13A N-terminal region
sufficient binding region
aa 1-606

coordinates relative to unc-13-PA

Rbp 
central region
sufficient binding region
aa 1040-1316

coordinates relative to Rbp-PF

Rbp 
SH3 domain III
sufficient binding region
aa 1441-1507

coordinates relative to Rbp-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Böhme et al., 2016)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Böhme et al., 2016)
FBrf0245633-7.Y2H
Reference
Petzoldt et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rbp 

RIM-BP

bait

fused to lexA DNA-binding domain

Rbp 
Rbp 
unc-13 

Unc13A

prey

fused to GAL4 activation domain

unc-13 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rbp 
SH3-II domain
sufficient binding region
aa 1040-1382

coordinates relative to Rbp-PF

Rbp 
SH3-III domain
sufficient binding region
aa 1441-1507

coordinates relative to Rbp-PF

unc-13 
PxxP domain
sufficient binding region
aa 1-606
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast lexA-BD/GAL4-AD. Positive control.

(Petzoldt et al., 2020)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Petzoldt et al., 2020)
FBrf0245633-8.Y2H
Reference
Petzoldt et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
unc-13 

Unc13A

bait

fused to lexA DNA-binding domain

unc-13 
Rbp 

RIM-BP

prey

fused to GAL4 activation domain

Rbp 
Rbp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rbp 
SH3-II domain
sufficient binding region
aa 1040-1382

coordinates relative to Rbp-PF

Rbp 
SH3-III domain
sufficient binding region
aa 1441-1507

coordinates relative to Rbp-PF

unc-13 
PxxP domain
sufficient binding region
aa 1-606
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Petzoldt et al., 2020)

Two-hybrid system: yeast lexA-BD/GAL4-AD. Positive control.

(Petzoldt et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)