Source was cell extract of COS cell line; bait produced from transfected construct; prey produced as a recombinant fusion protein in bacterial system.

Cells expressing tkv were incubated with radiolabeled BMP2, then treated with disuccinimdyl suberate cross-linker, which leads to radiolabeling of tkv. Dad was immunoprecipitated and autoradiography was used to detect coimmunoprecipitation of radiolabeled tkv.

Dad interacts with sax and tkv, but not with babo.

Source was cell extract of COS7 cell line; bait produced from transfected construct; prey produced from transfected construct.

Source was membrane fraction of S2 cell lysate; bait produced from transfected construct; prey produced from transfected construct.

A constitutively active form of tkv harboring a Q253D mutation was used in this assay.

Source was wing discs of transgenic fly line; proteins produced from tagged transgenic constructs.

Coexpression of Gli reduces the tkv-Dad interaction.

An un-tagged, constitutively active construct of tkv was used. Two-step coIP demonstrating IntS8 precipitates Dad and tkv in the first step. The first IP reactions was used for a second Dad coIP which demonstrated an interaction with tkv and IntS8, suggesting all three proteins associate.

Source was cell extract of S2 cell line; bait and prey produced from transfected constructs.