FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
lola, rib
FlyBase ID
FBig0000106652
Interaction Network
Interactions Browser links
lola network
rib network
Reported Interactions
FBrf0234063-3.Y2H
Reference
Silva et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
lola 

LOLA

bait

fused to GAL4 DNA-binding domain

lola 
rib 

RIB

prey

fused to GAL4 activation domain

rib 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
lola 
BTB domain
sufficient binding region
aa 1-124

coordinates relative to lola-PD

rib 
BTB domain
sufficient binding region
aa 1-135

coordinates relative to rib-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Silva et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Silva et al., 2016)
FBrf0234063-4.Y2H
Reference
Silva et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rib 

RIB

bait

fused to GAL4 DNA-binding domain

rib 
lola 

LOLA

prey

fused to GAL4 activation domain

lola 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
lola 
BTB domain
sufficient binding region
aa 1-124

coordinates relative to lola-PD

rib 
BTB domain
sufficient binding region
aa 1-135

coordinates relative to rib-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Silva et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Silva et al., 2016)
FBrf0260563-57.Y2H
Reference
Bonchuk et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
lola 

lola

bait

fused to GAL4 DNA-binding domain

lola 
rib 

Ribbon

prey

fused to GAL4 activation domain

rib 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
lola 
TTK-type BTB domain
sufficient binding region
aa 1-120
rib 
TTK-type BTB domain
sufficient binding region
aa 13-132
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bonchuk et al., 2024)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Bonchuk et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)