Source was cell extract of HeLa cell line; bait produced from transfected construct; prey produced from transfected construct.

Both whole cell lysates and membrane vesicles derived from HeLa cells were used in this experiment.

atl was assayed for its ability to mediate liposome fusion.

Interaction in vitro; enzyme produced as a recombinant fusion protein in bacterial system; enzyme target produced as a recombinant fusion protein in bacterial system.

GDP-bound form was crystallized.

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system.

atl was assayed for its ability to mediate liposome fusion.

Interaction in vitro; enzyme produced as a recombinant fusion protein in bacterial system; enzyme target produced as a recombinant fusion protein in bacterial system.

Donor and acceptor probes incorporated into an engineered T364C residue in the three helical bundle, which brings them within 30 angstroms, and enables visualization of G domain dimerization by FRET. Addition of GTP results in dimerization of wild-type and a D127N mutant, however the D127N mutant is unable undergo GTP hydrolysis, and is unable to disassemble.

Interaction in vitro; fluorescence donor produced from tagged transfected construct; fluorescence acceptor produced from tagged transfected construct.