Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).
Two-hybrid system: yeast LexA-BD/VP16-AD
construct "L3b"
construct "D", coordinates relative to shg-PA
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
