FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
osk, Tm1
FlyBase ID
FBig0000106857
Interaction Network
Interactions Browser links
osk network
Tm1 network
Reported Interactions
FBrf0234117-40.coIP.MS
Reference
Hurd et al., 2016
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

Osk

bait

FLAG tag

osk 
osk 
Tm1 

CG4898

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
long Osk isoform N-terminal region
sufficient binding region
aa 1-138

coordinates relative to osk-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Hurd et al., 2016)
FBrf0234661-3.XL.coIP.PCR
Reference
Gáspár et al., 2017
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tm1 

Tm1-I/C

bait

GFP tag

osk 

oskar

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Gáspár et al., 2017)
FBrf0234661-4.XL.coIP.PCR
Reference
Gáspár et al., 2017
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tm1 

Tm1-I/C

bait

GFP tag

osk 

oskar

prey

DIG label

osk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
3-prime UTR
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced and labeled by in vitro transcription.

(Gáspár et al., 2017)
FBrf0262977-5.EMSA
Reference
Gaber et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tm1 

Tm1

bait

GST tag

Tm1 
osk 

osk

prey

fluorescent dye label

osk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
3 prime UTR region 2 and 3
sufficient binding region
unspecified
Tm1 
interaction domain
sufficient binding region
aa 1-335
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

osk 3 prime UTR region 2 and 3 is a 534 nucleotide fragment.

(Gaber et al., 2025)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

(Gaber et al., 2025)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)