Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Yeast cells carried GAL1 and URA3 reporters under the control of the dl-regulated VRE regulatory element of the zen gene. These cells were transformed with dl and cDNA library to screen for factors that modulated dl-dependent activation of the reporters.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

Dsp1 and/or dl were mixed with [32]P labeled DNA and DNA-protein complexes observed by EMSA.