FlyBase Gene Report: Dmel\Dsp1

(Fisher et al., 2012)

embryonic stage 5

(Mosrin-Huaman et al., 1998)

embryonic stage 7 -- 8

(Fisher et al., 2012)

embryonic stage 9

(Mosrin-Huaman et al., 1998)

embryonic stage 9 -- 10

(Fisher et al., 2012)

embryonic stage 11 -- 12

larval central nervous system

(Fisher et al., 2012)

embryonic stage 13

(Mosrin-Huaman et al., 1998)

embryonic/larval anterior midgut

(Mosrin-Huaman et al., 1998)

embryonic/larval posterior midgut

(Mosrin-Huaman et al., 1998)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

(Fisher et al., 2012)

embryonic stage 14 -- 16

primordial germ cell

(Yatsu et al., 2008)

embryonic stage 16

larval ventral nerve cord

(Mosrin-Huaman et al., 1998)

larval brain

(Mosrin-Huaman et al., 1998)

Additional Descriptive Data

Dsp1 transcripts are first detected at the cellular blastoderm stage in every cell. During germband retraction, staining is localized to the epidermis and the CNS. At embryonic stage 15-16, Dsp1 transcripts are restricted to the ventral nerve cord and brain.

(Mosrin-Huaman et al., 1998)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval ventral nerve cord

(Mosrin-Huaman et al., 1998)

oogenesis stage S10

nurse cell

• cytoplasm

(Mosrin-Huaman et al., 1998)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

adult brain cell body rind

(Mosrin-Huaman et al., 1998)

larval stage

larval brain

(Mosrin-Huaman et al., 1998)

adult stage | female

ovary

(Mosrin-Huaman et al., 1998)

adult stage

adult head

(Mosrin-Huaman et al., 1998)

Additional Descriptive Data

Dsp1 protein is detected by western blot in ovary and adult head but not in testis, at all embryonic stages and in larval brain but not in Malpighian tubules, midgut, or salivary glands. Dsp1 protein is first detected by immunolocalization in egg chambers at oogenesis stage 10 where it is found in nurse cells in the cytoplasm. In third instar larvae it is observed at the extremity of the ventral ganglion but not in other parts of the brain.

(Mosrin-Huaman et al., 1998)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Mosrin-Huaman et al., 1998)

inferred from high throughput direct assay

(Lye et al., 2014)

located_in polytene chromosome

inferred from direct assay

(Lamiable et al., 2010)

Expression Deduced from Reporters

Reporter: PBac{CPTI-un}Dsp1^CPTI002844

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

Comment: cell bodies in adult brain cortex and glial sheath

(Knowles-Barley, 2011.8.24)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Dsp1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Dsp1

View exonic expression by tissue for Dmel\Dsp1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0011764

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

117294/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0278608

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011764

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0278608

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12223

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 11 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Dsp1

Transgenic constructs containing regulatory region of Dsp1

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(1)19

(Decoville et al., 2001)

Gene duplicated in

Dp(1;Y)shi⁺3

(Decoville et al., 2001)

Inferred from location ( 15 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^29BD

Dsp1^GS1115

lethal, with Scer\GAL4^Bx-MS1096

Dsp1^{UAS.ORF.GW.Tag:HA}

lethal, with Scer\GAL4^c355

Dsp1^GS1115

lethal, with Scer\GAL4^dpp.blk1

Dsp1^GS1115

lethal, with Scer\GAL4^hs.2sev

Dsp1^GS1115

lethal, with Scer\GAL4^pnr-MD237

Dsp1^GD4269

lethal | cold sensitive, with Scer\GAL4^Bx-MS1096

Dsp1^{UAS.ORF.GW.Tag:HA}

partially lethal

Dsp1¹

partially lethal | recessive

Dsp1¹

Sterility

Allele

semi-sterile

Dsp1¹

semi-sterile | recessive

Dsp1¹

Other Phenotypes

Allele

abnormal flight | recessive

Dsp1¹

abnormal locomotor behavior | recessive

Dsp1¹

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

Dsp1^EP355

visible, with Scer\GAL4^ap-md544

Dsp1^EP355

visible, with Scer\GAL4^GMR.PF

Dsp1^EP355

visible | adult stage | homeotic | recessive

Dsp1¹

visible | homeotic

Dsp1¹

wild-type

Dsp1²⁷⁴

Phenotype manifest in

Allele

abdominal 4 ventral denticle belt

Dsp1¹

abdominal 5 ventral denticle belt

Dsp1¹

abdominal sternite bristle | ectopic

Dsp1¹

adult abdominal segment 4

Dsp1¹

adult abdominal segment 5 | ectopic

Dsp1¹

adult abdominal segment 6

Dsp1¹

adult metathoracic segment anterior compartment

Dsp1¹

adult thorax | dorsal, with Scer\GAL4^ap-md544

Dsp1^EP355

anterior fascicle & synapse, with Scer\GAL4^elav-C155

Dsp1^EP355

egg

Dsp1¹

embryonic/first instar larval cuticle

Dsp1¹

eye, with Scer\GAL4^GMR.PF

Dsp1^EP355

haltere

Dsp1¹

larval intersegmental nerve, with Scer\GAL4^elav-C155

Dsp1^EP355

metathoracic laterotergite

Dsp1¹

metathoracic leg | ectopic

Dsp1¹

prothoracic leg

Dsp1¹

scutellum, with Scer\GAL4^ap-md544

Dsp1^EP355

scutum | ectopic

Dsp1¹

sex comb

Dsp1¹

wing, with Scer\GAL4^ap-md544

Dsp1^EP355

wing | ectopic

Dsp1¹

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (39)

Hsap\HMGB3

11 of 14

Yes

Hsap\HMGB1

10 of 14

Yes

Hsap\HMGB2

10 of 14

Yes

Hsap\HMGB1P1

8 of 14

Yes

Hsap\HMGB4

4 of 14

Yes

Hsap\SP100

3 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Hsap\UBTFL1

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (31)

Rnor\Hmgb3

10 of 14

Yes

Rnor\LOC108350839

10 of 14

Yes

Rnor\Hmg1l1

9 of 14

Yes

Rnor\Hmgb1

9 of 14

Yes

Rnor\Hmgb2

9 of 14

Yes

Rnor\LOC108349189

9 of 14

Yes

Rnor\RGD1559962

9 of 14

Yes

Rnor\RGD1563668

9 of 14

Yes

Rnor\Hmgb2l1

8 of 14

Yes

Rnor\RGD1561694

8 of 14

Yes

Rnor\Hmgb4

4 of 14

Yes

Rnor\LOC681067

3 of 14

Yes

Rnor\LOC691030

3 of 14

Yes

Rnor\LOC108349258

3 of 14

Yes

Rnor\RGD1560585

3 of 14

Yes

Rnor\RGD1561558

3 of 14

Yes

Rnor\RGD1566225

3 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Rnor\Hmg20b

1 of 14

Rnor\Hmgb1-ps3

1 of 14

Yes

Rnor\LOC690521

1 of 14

Yes

Rnor\Pbrm1

1 of 14

Rnor\RGD1565762

1 of 14

Yes

Rnor\Smarce1

1 of 14

Rnor\Ssrp1

1 of 14

Mus musculus (laboratory mouse) (15)

Mmus\Hmgb1

10 of 14

Yes

Mmus\Hmgb2

10 of 14

Yes

Mmus\Hmgb3

10 of 14

Yes

Mmus\Hmgb4

5 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Mmus\Ubtf

2 of 14

Yes

Mmus\Hmg20b

1 of 14

Mmus\Smarce1

1 of 14

Mmus\Ssrp1

1 of 14

Xenopus tropicalis (Western clawed frog) (29)

Xtro\hmgb1

8 of 13

Yes

Xtro\hmgb2

8 of 13

Yes

Xtro\hmgb3

8 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (22)

Drer\hmgb2a

11 of 14

Yes

Drer\hmgb3a

11 of 14

Yes

Drer\hmgb3b

11 of 14

Yes

Drer\hmgb1b

10 of 14

Yes

Drer\hmgb1a

9 of 14

Yes

Drer\hmgb2b

9 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Drer\ubtfl

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (16)

Cele\hmg-1.2

9 of 14

Yes

4 of 14

3 of 14

2 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (17)

Agam\AgaP_AGAP000005

11 of 12

Yes

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (15)

Atha\3xHMG-box1

7 of 13

Yes

Atha\3xHMG-box2

7 of 13

Yes

6 of 13

6 of 13

6 of 13

5 of 13

4 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\IXR1

7 of 13

Yes

Scer\NHP6B

7 of 13

Yes

Scer\NHP6A

6 of 13

Yes

Scer\ABF2

4 of 13

Scer\NHP10

4 of 13

Yes

Scer\HMO1

3 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\nhp6

7 of 12

Yes

Spom\cmb1

3 of 12

Yes

Spom\SPBC28F2.11

3 of 12

Yes

Spom\nht1

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Dsp1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (19)

6 of 13

4 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

HMGB3; high mobility group box 3

model of syndromic microphthalmia 13

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Dsp1^EP355

exacerbates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.GMR.Tag:SS(rPENK)}

(Cao et al., 2008)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

HMGB3; high mobility group box 3

11 of 14

300193

MICROPHTHALMIA, SYNDROMIC 13; MCOPS13

syndromic microphthalmia 13

HMGB1; high mobility group box 1

10 of 14

163905

HMGB2; high mobility group box 2

10 of 14

163906

HMGB1P1; high mobility group box 1 pseudogene 1

8 of 14

HMGB4; high mobility group box 4

4 of 14

617285

SP100; SP100 nuclear antigen

3 of 14

604585

TFAM; transcription factor A, mitochondrial

3 of 14

600438

MITOCHONDRIAL DNA DEPLETION SYNDROME 15 (HEPATOCEREBRAL TYPE); MTDPS15

mitochondrial DNA depletion syndrome 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Dsp1 - Dll

anti bait coimmunoprecipitation, western blot

(Hu et al., 2017.6.13)

Dsp1 - Dref

two hybrid array

(Shokri et al., 2019)

Dsp1 - HDAC1

(Kim et al., 2007)

Dsp1 - Jra

anti bait coimmunoprecipitation, western blot

(Kim et al., 2007)

Dsp1 - Lim1

cosedimentation through density gradient, western blot, anti bait coimmunoprecipitation, Identification by mass spectrometry, anti tag coimmunoprecipitation

(Hu et al., 2017.6.13)

Dsp1 - Rm62

(Lamiable et al., 2010)

Dsp1 - Sag1

anti bait coimmunoprecipitation, western blot

(Hu et al., 2017.6.13)

Dsp1 - Stat92E

(Kim et al., 2007)

Dsp1 - corto

anti bait coimmunoprecipitation, western blot, far western blotting, autoradiography, pull down

(Salvaing et al., 2006)

Dsp1 - dl

far western blotting, autoradiography, pull down, one hybrid, electrophoretic mobility shift assay

(Decoville et al., 2000, Lehming et al., 1994)

Dsp1 - ens

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Dsp1 - psq

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Chetverina et al., 2022)

RNA-protein

Physical Interaction

Assay

References

Dsp1 - Ythdc1

(Knuckles et al., 2018, Lence et al., 2016)

Dsp1 - fl(2)d

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Knuckles et al., 2018)

Dsp1 - nito

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Knuckles et al., 2018)

Dsp1 - stau

anti tag coimmunoprecipitation, partial DNA sequence identification by hybridization, anti bait coimmunoprecipitation

(Laver et al., 2013)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

ash1

(Decoville et al., 2001)

suppressible

(Decoville et al., 2001)

enhanceable

Scr

(Decoville et al., 2001)

enhanceable

trx

(Decoville et al., 2001)

enhanceable

Ubx

(Decoville et al., 2001)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Antp

suppressible

(Decoville et al., 2001)

btl|stumps

suppressible

(Zhu et al., 2005)

suppressible

(Müller et al., 2005)

enhanceable

(Decoville et al., 2001)

suppressible

(Decoville et al., 2001)

pnr

enhanceable

(Pena-Rangel et al., 2002)

enhanceable

(Zhu et al., 2005)

suppressible

(Decoville et al., 2001)

Scr

enhanceable

(Decoville et al., 2001)

vtd

suppressible