FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Keap1, cnc
FlyBase ID
FBig0000106922
Interaction Network
Interactions Browser links
Keap1 network
cnc network
Reported Interactions
FBrf0225821-1.BiFC.FM
Reference
Deng and Kerppola, 2014
Description
physical association
Assay
Collection
Source/Stage
embryonic/larval salivary gland / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Keap1 

Keap1

bait

YFP tag, N-terminal fragment

cnc 

cnc

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval salivary gland of transgenic fly line; proteins produced from tagged transgenic constructs.

(Deng and Kerppola, 2014)
FBrf0225821-4.BiFC.FM
Reference
Deng and Kerppola, 2014
Description
physical association
Assay
Collection
Source/Stage
embryonic/larval salivary gland / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cnc 

cnc

bait

YFP tag, N-terminal fragment

Keap1 

Keap1

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval salivary gland of transgenic fly line; proteins produced from tagged transgenic constructs.

(Deng and Kerppola, 2014)
FBrf0228687-7.PD.A
Reference
Jain et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Keap1 

DmKeap1

bait

GST tag

cnc 

Cnc

prey

[35]S label

cnc 
cnc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
cnc 
N-terminal region
necessary binding region
aa 1-464

coordinates relative to cnc-PC

cnc 
ETGE motif residues 460-462
mutation disrupting interaction
aa 460-462

E460A, G462A, coordinates relative to cnc-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Jain et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)