FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
kni, gro
FlyBase ID
FBig0000107098
Interaction Network
Interactions Browser links
kni network
gro network
Reported Interactions
FBrf0208965-1.PD.WB
Reference
Payankaulam and Arnosti, 2009
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kni 

Kni

bait

His tag, FLAG tag

gro 

Gro

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Payankaulam and Arnosti, 2009)
FBrf0208965-2.coIP.WB
Reference
Payankaulam and Arnosti, 2009
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kni 

Kni

bait

FLAG tag, His tag

gro 

Gro

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Payankaulam and Arnosti, 2009)
FBrf0208965-4.PD.A
Reference
Payankaulam and Arnosti, 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kni 

Kni

bait

GST tag

kni 
kni 
kni 
gro 

Gro

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
kni 
interaction domain
sufficient binding region
aa 139-330

coordinates relative to kni-PA

kni 
interaction domain
necessary binding region
aa 169-189

"169-189Δ" construct, coordinates relative to kni-PA

kni 
eh1 motif
necessary binding region
aa 85-91

"169-189Δ" construct, coordinates relative to kni-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Payankaulam and Arnosti, 2009)

Two independent regions of kni mediate binding to gro.

(Payankaulam and Arnosti, 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)