Sp1 binds directly to Taf4 but not Taf1 or Taf2.

Sp1 was bound to a column containing biotinylated DNA bearing Sp1-binding sites. This column was then incubated with recombinant Taf4.

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected insect cell system; prey produced as a recombinant fusion protein in baculovirus-infected insect cell system.