Dpp
HA tag
Tsg
His tag
Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.
dpp, scw and tsg were cotransfected and purified from conditioned medium.
dpp
tsg
Interaction in vitro; bait and prey produced and purified from S2 transfected cells.
Addition of sog increases interaction.