Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Isoform-specific antibody used to immunoprecipitate lola.
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
