FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
brp, unc-13
FlyBase ID
FBig0000107665
Interaction Network
Interactions Browser links
brp network
unc-13 network
Reported Interactions
FBrf0233590-2.Y2H
Reference
Böhme et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
brp 

BRP

bait

fused to LexA DNA-binding domain

brp 
unc-13 

Unc13A

prey

fused to GAL4 activation domain

unc-13 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
unc-13 
Unc13A N-terminal region
sufficient binding region
aa 1-606

coordinates relative to unc-13-PA

brp 
N-terminal region
sufficient binding region
aa 100-650

coordinates relative to brp-PG

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Böhme et al., 2016)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Böhme et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)