FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
tra2, dsx
FlyBase ID
FBig0000107695
Interaction Network
Interactions Browser links
tra2 network
dsx network
Reported Interactions
FBrf0053383-1.XL.A
Reference
Hedley and Maniatis, 1991
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dsx 

dsx

bait

[32]P label

dsx 
dsx 
tra2 

tra-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dsx 
female-specific terminal exon
sufficient binding region

FspI-FspI fragment

dsx 
dsx 13-nt repeat 5
sufficient binding region
GCACGCCAAAUCAACGAUCAACAUUUCAACACG

three tandem copies of the UCAACGAUCAACA with 10 nt flanking sequence on either side

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.

(Hedley and Maniatis, 1991)
FBrf0053383-2.EMSA.A
Reference
Hedley and Maniatis, 1991
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tra2 

tra-2

bait
dsx 

dsx

prey

[32]P label

dsx 
dsx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dsx 
female-specific terminal exon
sufficient binding region

FspI-FspI fragment

dsx 
dsx 13-nt repeat 5
sufficient binding region
GCACGCCAAAUCAACGAUCAACAUUUCAACACG

three tandem copies of the UCAACGAUCAACA with 10 nt flanking sequence on either side

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.

(Hedley and Maniatis, 1991)
FBrf0057558-1.XL.A
Reference
Inoue et al., 1992
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dsx 

dsx

bait

[32]P label

dsx 
dsx 
tra2 

tra-2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dsx 
dsx 13-nt repeat A
sufficient binding region
UCUUCAAUCAACA

two tandem repeats used

dsx 
dsx 13-nt repeat C
sufficient binding region
UCAACAAUCAACA

two tandem repeats used

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.

(Inoue et al., 1992)
FBrf0068398-1.XL.A
Reference
Amrein et al., 1994
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dsx 

dsx

bait

[32]P label

dsx 
tra2 

Tra2

prey
tra2 
tra2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tra2 
RNP-CS domain
necessary binding region
aa 78-160

"D6" construct, coordinates relative to tra2-PA

tra2 
RS2 domain
necessary binding region
aa 201-235

"D4" construct, coordinates relative to tra2-PA

dsx 
splice regulatory element 13-nt repeats
sufficient binding region

"B" fragment

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced as a recombinant fusion protein in bacterial system.

(Amrein et al., 1994)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)