FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Ced-12, mbc
FlyBase ID
FBig0000107751
Interaction Network
Interactions Browser links
Ced-12 network
mbc network
Reported Interactions
FBrf0180198-7.coIP.WB
Reference
Ishimaru et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mbc 

Mbc

bait

HA tag

mbc 
Ced-12 

ELMO

prey

Myc tag

Ced-12 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mbc 
SH3 domain
necessary binding region
aa 1-70

"ΔSH3" construct, coordinates relative to mbc-PA

Ced-12 
proline rich domain
necessary binding region
aa 708-724

"ΔPRM" construct, coordinates relative to Ced-12-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Ishimaru et al., 2004)
FBrf0202723-1.Y2H
Reference
Geisbrecht et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mbc 

MBC

bait

fused to GAL4 DNA-binding domain

mbc 
Ced-12 

ELMO

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mbc 
SH3 domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Geisbrecht et al., 2008)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Geisbrecht et al., 2008)
FBrf0202723-2.Y2H
Reference
Geisbrecht et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mbc 

MBC

bait

fused to GAL4 DNA-binding domain

mbc 
Ced-12 

ELMO

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mbc 
interaction domain
sufficient binding region
aa 1-1807
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Geisbrecht et al., 2008)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Geisbrecht et al., 2008)
FBrf0202723-3.coIP.WB
Reference
Geisbrecht et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mbc 

MBC

bait

HA tag

Ced-12 

ELMO

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Geisbrecht et al., 2008)
FBrf0202723-4.coIP.WB
Reference
Geisbrecht et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ced-12 

ELMO

bait

HA tag

mbc 

MBC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Geisbrecht et al., 2008)
FBrf0202723-5.coIP.MS
Reference
Geisbrecht et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mbc 

MBC

bait

HA tag

Ced-12 

ELMO

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Geisbrecht et al., 2008)
FBrf0202723-6.coIP.MS
Reference
Geisbrecht et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ced-12 

ELMO

bait

HA tag

mbc 

MBC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Geisbrecht et al., 2008)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)