FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000107890
Interaction Network
Interactions Browser links
dia network
ena network
Reported Interactions
FBrf0209542-1.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ena 

Ena

bait

GFP tag

dia 

Dia

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

FBrf0209542-2.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dia 

Dia

bait
ena 

Ena

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

FBrf0224233-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dia 

dia

bait

fused to lexA DNA-binding domain

dia 
ena 

ena

prey

fused to B42 activation domain

ena 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dia 
FH1 domain
sufficient binding region
aa 501-598

coordinates relative to dia-PA

ena 
EVH1 domain
sufficient binding region
aa 1-112

coordinates relative to ena-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Interaction with full-length proteins not detected in this assay.

FBrf0224233-2.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ena 

ena

bait

fused to lexA DNA-binding domain

ena 
dia 

dia

prey

fused to B42 activation domain

dia 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dia 
FH1FH2 domain
sufficient binding region
aa 501-995

coordinates relative to dia-PA

ena 
EVH1 domain
sufficient binding region
aa 1-112

coordinates relative to ena-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Interaction with full-length proteins not detected in this assay.

FBrf0224233-3.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dia 

dia

bait

GST tag

dia 
ena 

ena

prey
ena 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dia 
FH1 domain
sufficient binding region
aa 501-598

coordinates relative to dia-PA

ena 
EVH1 domain
sufficient binding region
aa 1-112

coordinates relative to ena-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0224233-4.BiFC.FM
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dia 

dia

bait

YFP tag, N-terminal fragment

dia 
ena 

ena

prey

YFP tag, C-terminal fragment

ena 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dia 
FH1FH2 domain
sufficient binding region
aa 501-995

coordinates relative to dia-PA

ena 
EVH1 domain
sufficient binding region
aa 1-112

coordinates relative to ena-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live D16 cells; proteins produced from transfected constructs.

FBrf0224233-5.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ena 

ena

bait

GST tag

ena 
ena 
dia 

dia

prey
dia 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
dia 
FH1 domain
sufficient binding region
aa 501-598

coordinates relative to dia-PA

ena 
EVH1 domain
sufficient binding region
aa 1-112

coordinates relative to ena-PD

ena 
EVH1 domain residue 77
mutation disrupting interaction
aa 77

F77E, coordinates relative to ena-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)