Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Source was embryos of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Interaction with full-length proteins not detected in this assay.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Interaction with full-length proteins not detected in this assay.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Source was live D16 cells; proteins produced from transfected constructs.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.