FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
comt, Nrx-1
FlyBase ID
FBig0000108240
Interaction Network
Interactions Browser links
comt network
Nrx-1 network
Reported Interactions
FBrf0229008-1.Y2H
Reference
Li et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nrx-1 

NRX

bait

fused to GAL4 DNA-binding domain

Nrx-1 
comt 

comt

prey

fused to GAL4 activation domain

comt 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nrx-1 
intracellular domain
sufficient binding region
aa 1716-1837

coordinates relative to Nrx-1-PA

comt 
C-terminal region
sufficient binding region
aa 351-745

coordinates relative to comt-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Li et al., 2015)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from cDNA expression library).

(Li et al., 2015)
FBrf0229008-2.coIP.WB
Reference
Li et al., 2015
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nrx-1 

Nrx

bait
comt 

NSF

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Li et al., 2015)
FBrf0229008-3.PD.WB
Reference
Li et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
comt 

NSF

bait

GST tag

comt 
Nrx-1 

Nrx

prey

His tag

Nrx-1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nrx-1 
intracellular domain
sufficient binding region
aa 1716-1813aa 1787-1813

construct "d", coordinates relative to Nrx-1-PA

construct "f", coordinates relative to Nrx-1-PA

comt 
C-terminal region
necessary binding region
aa 513-588

construct "3", coordinates relative to comt-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

comt binds Nrx-1 with affinity of Kd = 4.4uM.

(Li et al., 2015)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Li et al., 2015)
FBrf0229008-4.PD.WB
Reference
Li et al., 2015
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Nrx-1 

Nrx

bait

GST tag

Nrx-1 
comt 

NSF

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Nrx-1 
intracellular domain
sufficient binding region
aa 1716-1837

coordinates relative to Nrx-1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult head extract.

(Li et al., 2015)

Nrx-1 binding to comt is modulated by [Ca++] - optimal [Ca++] is 0.5mM.

(Li et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)