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FB2026_01
,
released March 12, 2026
dNSF1, NSF1, NSF, com, dNSF-1
homohexameric AAA ATPase - developmentally regulated N-ethylmaleimide-sensitive fusion (NSF) gene - mutants are defective in the synaptic growth and maturation that occurs during larval development - functions to recycle SNARE complex components subsequent to membrane fusion - required for tracheal tube growth and connectivity within the terminal cell
Please see the JBrowse view of Dmel\comt for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.44
Gene model reviewed during 5.39
Gene model reviewed during 5.43
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
Gene model reviewed during 5.50
3.2 (northern blot)
745 (aa)
Homohexamer.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\comt using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: maternally deposited
Comment: reported as dorsal/lateral sensory complexes
comt transcripts are detected in RNA from larvae and adults on northern blots. In situ hybridization in embryos shows expression throughout the embryo with strong staining in the CNS.
comt protein is enriched in the neuropil of the larval ventral ganglion, where synapses occur.
JBrowse - Visual display of RNA-Seq signals
View Dmel\comt in JBrowse
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
comt and Nsf2 exhibit similar functional properties but have evolved distinct tissue-specific roles. comt is required in the nervous system beginning at the adult stage of development whereas Nsf2 is required in the mesoderm beginning at the first larval instar. The comt and Nsf2 proteins can functionally substitute for one another.
comt normally dissociates and recycles SNARE proteins during the interval between exocytosis and endocytosis in the synaptic vesicle cycle.
Paralysis in comt mutant flies is activity dependent.
Evidence of multiple, highly related isoforms of NSF in a single species suggests that in some tissue membranes fusion and/or secretion has a specific role during development.
Mutations of comt are defective in one component of the molecular machinery mediating vesicle targetting and fusion, N-ethylmaleimide-sensitive fusion protein (NSF). comt exhibits an apparent defect in synaptic transmission which is the first functional evidence that NSF in involved in this process.
comt gene has been cloned and the gene product characterised.
The neurosecretory apparatus of the presynaptic nerve terminal contains a core complex of conserved secretory proteins, comt is a component this complex.
Larvae or adults become paralyzed when exposed to high temperatures, but this requires many seconds for induction; recovery from immobility on lowering of temperature is slow and directly correlated with duration of paralysis; most of the comt alleles do not induce relatively rapid paralysis until 38oC (Siddiqi and Benzer, 1976), but two (comt2, comt3) cause pass-out at 29oC (Homyk, Szidonya and Suzuki, 1980); the mutations induce graded decrease and recovery of function of synapses at neuromuscular junctions as temperature is raised and lowered (Siddiqi and Benzer, 1976); several alleles are cold-sensitive for paralysis as well as temperature-sensitive (Siddiqi and Benzer, 1976). ERG normal (Homyk and Pye, 1989).
Source for merge of: comt CG1618
