Subject: Re: Question about comt alleles
>Dear Dr. Littleton,
>
>I am a curator for FlyBase and am currently capturing the genomic sites
>of the comt mutations that you reported in your 2001 PNAS paper, to
>appear in the genome maps in FlyBase. I have a question about the
>comtG8 and comt1121 alleles.
>
>comtG8 is reported as T464@. The T464 codon is ACC and since it would
>require nucleotide changes at all three positions of the codon to get a
>stop, I wondered if you could give me further details about the nature
>of this mutation.
G8 was a gamma ray induced comt allele.
>
>comt1121 is reported as P494L, however the amino acid codon at
>position 494 in the sequenced strain is a Thr (ACG) and not Pro, so I
wanted to check on that mutation as well.
I'll have to check on this one, as its been almost 10 years since it 
was sequenced.  It may require going back to Barry and seeing if he 
still has the old notes on its generation in his lab, as it was a 
preexisting mutant.
Regards,
Troy
-- 
J. Troy Littleton, Ph.D., M.D
Associate Professor of Neurobiology
The Picower Center for Learning and Memory
Department of Biology
Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology
50 Ames St.
E18-672
Cambridge, MA 02139